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Pass the capillary through the heater coil without touching it.

In the world of electrophysiology,显微注射 (microinjection), and patch-clamp techniques, the vertical micropipette puller is legendary. For decades, this robust, gravity-based puller was a staple in neurobiology and developmental biology labs worldwide. Despite newer digital models flooding the market, thousands of PC-10 units are still in active use—humming away on lab benches from Tokyo to Boston.

Locate the top and bottom slider clamps. Loosen the thumbscrews, slide the glass capillary through the center of the platinum heating coil, and secure it firmly inside both the upper and lower clamping blocks. Ensure the glass is perfectly centered within the loop of the heating coil. Step 3: Configure Your Stages

Adjust the dial to a slightly lower or higher setting depending on the desired taper smoothness.

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Narishige PC-10 Glass Pipette Puller: Setup, Operation, and Maintenance Manual

List the when pulling thin-walled glass.

Illuminates when current is actively flowing through the heating coil.

Shield the unit from room air currents and clean the vertical guide shafts.

Realign the platinum coil so the glass capillary passes directly through its true center. Air drafts or dirty sliding rails.